Explore Workflows
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scatter-wf3_v1_2.cwl#main
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Path: testdata/scatter-wf3_v1_2.cwl Branch/Commit ID: c1875d54dedc41b1d2fa08634dcf1caa8f1bc631 Packed ID: main |
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Motif Finding with HOMER with custom background regions
Motif Finding with HOMER with custom background regions --------------------------------------------------- HOMER contains a novel motif discovery algorithm that was designed for regulatory element analysis in genomics applications (DNA only, no protein). It is a differential motif discovery algorithm, which means that it takes two sets of sequences and tries to identify the regulatory elements that are specifically enriched in on set relative to the other. It uses ZOOPS scoring (zero or one occurrence per sequence) coupled with the hypergeometric enrichment calculations (or binomial) to determine motif enrichment. HOMER also tries its best to account for sequenced bias in the dataset. It was designed with ChIP-Seq and promoter analysis in mind, but can be applied to pretty much any nucleic acids motif finding problem. For more information please refer to: ------------------------------------- [Official documentation](http://homer.ucsd.edu/homer/motif/) |
Path: workflows/homer-motif-analysis-bg.cwl Branch/Commit ID: 7030da528559c7106d156284e50ff0ecedab0c4e |
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bam to trimmed fastqs and HISAT alignments
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Path: definitions/subworkflows/bam_to_trimmed_fastq_and_hisat_alignments.cwl Branch/Commit ID: c6bbd4cdd612b3b5cc6e9000df4800c21e192bf5 |
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mut3.cwl
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Path: tests/wf/mut3.cwl Branch/Commit ID: 2dce710246e091f0189fab41b589ee062ee94500 |
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Bacterial Annotation, pass 4, blastp-based functional annotation (second pass)
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Path: bacterial_annot/wf_bacterial_annot_pass4.cwl Branch/Commit ID: 8af4e2aabf43d5e3c7162efae4ad4649df5601e2 |
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RNA-Seq pipeline single-read strand specific
Note: should be updated The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for **strand specific single-read** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-read RNA-Seq data. It performs the following steps: 1. Use STAR to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. Use fastx_quality_stats to analyze input FASTQ file and generate quality statistics file 3. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
Path: workflows/rnaseq-se-dutp.cwl Branch/Commit ID: 9ee330737f4603e4e959ffe786fbb2046db70a00 |
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cache_asnb_entries
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Path: task_types/tt_cache_asnb_entries.cwl Branch/Commit ID: e71779665f42fcf34601b0f65e030bb0dd47fa79 |
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workflow_input_sf_expr_v1_1.cwl
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Path: testdata/workflow_input_sf_expr_v1_1.cwl Branch/Commit ID: c1875d54dedc41b1d2fa08634dcf1caa8f1bc631 |
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Trim Galore RNA-Seq pipeline paired-end
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **pair-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-end RNA-Seq data. It performs the following steps: 1. Trim adapters from input FASTQ files 2. Use STAR to align reads from input FASTQ files according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 3. Use fastx_quality_stats to analyze input FASTQ files and generate quality statistics files 4. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ files to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
Path: workflows/trim-rnaseq-pe.cwl Branch/Commit ID: c602e3cdd72ff904dd54d46ba2b5146eb1c57022 |
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kmer_build_tree
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Path: task_types/tt_kmer_build_tree.cwl Branch/Commit ID: 72804b6506c9f54ec75627f82aafe6a28d7a49fa |
