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Cell Ranger Count (ATAC)
Cell Ranger Count (ATAC) Quantifies single-cell chromatin accessibility of the sequencing data from a single 10x Genomics library. The results of this workflow are used in either the “Single-Cell ATAC-Seq Filtering Analysis” or “Cell Ranger Aggregate (ATAC)” pipeline. |
Path: workflows/cellranger-atac-count.cwl Branch/Commit ID: 93b844a80f4008cc973ea9b5efedaff32a343895 |
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Exome QC workflow
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Path: definitions/subworkflows/qc_exome.cwl Branch/Commit ID: 0a9a4ce83b49ed4e7eee5bcc09d83725136a36b0 |
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heatmap-prepare.cwl
Workflow runs homer-make-tag-directory.cwl tool using scatter for the following inputs - bam_file - fragment_size - total_reads `dotproduct` is used as a `scatterMethod`, so one element will be taken from each array to construct each job: 1) bam_file[0] fragment_size[0] total_reads[0] 2) bam_file[1] fragment_size[1] total_reads[1] ... N) bam_file[N] fragment_size[N] total_reads[N] `bam_file`, `fragment_size` and `total_reads` arrays should have the identical order. |
Path: tools/heatmap-prepare.cwl Branch/Commit ID: a8909e86f5bcb048d136f9a7d70b4b6f8f4e485f |
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Unaligned BAM to BQSR and VCF
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Path: definitions/subworkflows/bam_to_bqsr.cwl Branch/Commit ID: 0a9a4ce83b49ed4e7eee5bcc09d83725136a36b0 |
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RNA-Seq pipeline paired-end strand specific
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **paired-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the paired-end RNA-Seq data. It performs the following steps: 1. Use STAR to align reads from input FASTQ files according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. Use fastx_quality_stats to analyze input FASTQ files and generate quality statistics files 3. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 4. Generate BigWig file on the base of sorted BAM file 5. Map input FASTQ files to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 6. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
Path: workflows/rnaseq-pe-dutp.cwl Branch/Commit ID: a68821bf3a9ceadc3b2ffbb535d601d9a645b377 |
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scatter-wf1.cwl
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Path: v1.0/v1.0/scatter-wf1.cwl Branch/Commit ID: 4fd45edb9531a03223c18a586e32d0baf0d5acb2 |
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assemble.cwl
Assemble a set of reads using SKESA |
Path: assemble.cwl Branch/Commit ID: 77a9fa25b89ce73582a1ce6ba75fa6d2537fb8e8 |
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Xenbase ChIP-Seq pipeline paired-end
1. Convert input SRA file into pair of upsrtream and downstream FASTQ files (run fastq-dump) 2. Analyze quality of FASTQ files (run fastqc with each of the FASTQ files) 3. If any of the following fields in fastqc generated report is marked as failed for at least one of input FASTQ files: \"Per base sequence quality\", \"Per sequence quality scores\", \"Overrepresented sequences\", \"Adapter Content\", - trim adapters (run trimmomatic) 4. Align original or trimmed FASTQ files to reference genome (run Bowtie2) 5. Sort and index generated by Bowtie2 BAM file (run samtools sort, samtools index) 6. Remove duplicates in sorted BAM file (run picard) 7. Sort and index BAM file after duplicates removing (run samtools sort, samtools index) 8. Count mapped reads number in sorted BAM file (run bamtools stats) 9. Generate genome coverage BED file (run bedtools genomecov) 10. Sort genearted BED file (run sort) 11. Generate genome coverage bigWig file from BED file (run bedGraphToBigWig) |
Path: workflows/xenbase-chipseq-pe.cwl Branch/Commit ID: 9ee330737f4603e4e959ffe786fbb2046db70a00 |
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no-inputs-wf.cwl
Workflow without inputs. |
Path: tests/no-inputs-wf.cwl Branch/Commit ID: 57baec040c99d7edef8242ef51b5470b1c82d733 |
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count-lines13-wf.cwl
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Path: tests/count-lines13-wf.cwl Branch/Commit ID: 57baec040c99d7edef8242ef51b5470b1c82d733 |
