Workflow: Chipseq alignment for mouse with qc and creating homer tag directory
Fetched 2023-01-10 09:20:00 GMT
Verified with cwltool version 3.1.20221201130942
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- Inputs/Outputs
This workflow is Open Source and may be reused according to the terms of:
MIT License
Note that the tools invoked by the workflow may have separate licenses.
Inputs
| ID | Type | Title | Doc |
|---|---|---|---|
| reference | File | ||
| final_name | String (Optional) | ||
| chipseq_sequence | https://w3id.org/cwl/view/git/233f026ffce240071edda526391be0c03186fce8/definitions/types/sequence_data.yml#sequence_data[] | ||
| summary_intervals | https://w3id.org/cwl/view/git/233f026ffce240071edda526391be0c03186fce8/definitions/types/labelled_file.yml#labelled_file[] | ||
| per_base_intervals | https://w3id.org/cwl/view/git/233f026ffce240071edda526391be0c03186fce8/definitions/types/labelled_file.yml#labelled_file[] | ||
| minimum_base_quality | Integer (Optional) | ||
| per_target_intervals | https://w3id.org/cwl/view/git/233f026ffce240071edda526391be0c03186fce8/definitions/types/labelled_file.yml#labelled_file[] | ||
| minimum_mapping_quality | Integer (Optional) | ||
| picard_metric_accumulation_level | String |
Steps
| ID | Runs | Label | Doc |
|---|---|---|---|
| qc |
../subworkflows/qc_wgs_mouse.cwl
(Workflow)
|
WGS QC workflow mouse | |
| alignment |
../subworkflows/sequence_to_bqsr_mouse.cwl
(Workflow)
|
Alignment without BQSR | |
| bam_to_sam |
../tools/bam_to_sam.cwl
(CommandLineTool)
|
BAM to SAM conversion | |
| homer_tag_directory |
../tools/homer_tag_directory.cwl
(CommandLineTool)
|
Creating the homer tag directory |
Outputs
| ID | Type | Label | Doc |
|---|---|---|---|
| bam | File | ||
| flagstats | File | ||
| wgs_metrics | File | ||
| tag_directory | Directory | ||
| gc_bias_metrics | File | ||
| bamcoverage_bigwig | File | ||
| summary_hs_metrics | File[] | ||
| insert_size_metrics | File | ||
| per_base_hs_metrics | File[] | ||
| gc_bias_metrics_chart | File | ||
| insert_size_histogram | File | ||
| per_target_hs_metrics | File[] | ||
| gc_bias_metrics_summary | File | ||
| mark_duplicates_metrics | File | ||
| alignment_summary_metrics | File | ||
| per_base_coverage_metrics | File[] | ||
| per_target_coverage_metrics | File[] |
Permalink:
https://w3id.org/cwl/view/git/233f026ffce240071edda526391be0c03186fce8/definitions/pipelines/chipseq_alignment_mouse.cwl
