Workflow: Short read alignment pipeline

Fetched 2023-01-12 15:37:11 GMT
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Inputs

ID Type Title Doc
nthreads Integer Number of threads for alignment
fastq_dir Directory Directory containing two FASTQ files
hisat2_idx_basedir Directory HISAT2 index directory
hisat2_idx_basename String HISAT2 index basename

Steps

ID Runs Label Doc
sort_bam
alignment-steps/sort-bam.cwl (CommandLineTool)
Sort BAM file
align_reads
alignment-steps/align-reads.cwl (CommandLineTool)
convert_to_bam
alignment-steps/convert-to-bam.cwl (CommandLineTool)
Convert SAM to BAM

Outputs

ID Type Label Doc
output_bam File Sorted BAM file
Permalink: https://w3id.org/cwl/view/git/eb4c7911d3a6d9fc929eda11e1e03d74b0469cb9/alignment-pipeline.cwl