Workflow: SPRM pipeline

Fetched 2026-10-11 12:08:57 GMT
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Inputs

ID Type Title Doc
verbose Boolean (Optional) Whether to enable verbose/debug mode
mask_dir Directory Directory containing mask files
image_dir Directory Directory containing image files
processes Integer (Optional) Number of images to process in parallel (default: 1)
min_memory Boolean (Optional) Whether to conserve memory by leaving large arrays on disk
options_file File (Optional) SPRM options file
enable_manhole Boolean (Optional) Whether to enable remote debugging via 'manhole'
options_preset String (Optional) SPRM options preset (alternate options file bundled with the package)
threadpool_limit Integer (Optional) Whether to enable the Python 'faulthandler' module
enable_faulthandler Boolean (Optional) Whether to enable the Python 'faulthandler' module
cell_types_directory Directory[] (Optional) Cell type annotations file

Steps

ID Runs Label Doc
sprm
steps/sprm.cwl (CommandLineTool)
SPRM analysis
create_spatial_data
steps/create-spatial-data.cwl (CommandLineTool)
SPRM analysis
ome_tiff_normalize_expr
ome-tiff-normalize/ome_tiff_normalize.cwl (CommandLineTool)
OME-TIFF metadata normalization via bftools
ome_tiff_normalize_mask
ome-tiff-normalize/ome_tiff_normalize.cwl (CommandLineTool)
OME-TIFF metadata normalization via bftools

Outputs

ID Type Label Doc
sprm_output Directory SPRM output
sdata_zarr_zips File[]
segmentation_metadata_json File
Permalink: https://w3id.org/cwl/view/git/002cd44249383171312b6df85cebc247e87b606d/pipeline.cwl