Workflow: umi molecular alignment workflow
Fetched 2023-01-05 01:18:03 GMT
Verified with cwltool version 3.1.20221201130942
- Selected
- |
- Default Values
- Nested Workflows
- Tools
- Inputs/Outputs
This workflow is Open Source and may be reused according to the terms of:
MIT License
Note that the tools invoked by the workflow may have separate licenses.
Inputs
| ID | Type | Title | Doc |
|---|---|---|---|
| bam | File[] | ||
| reference | String | ||
| sample_name | String | ||
| read_structure | String[] | ||
| target_intervals | File (Optional) |
Steps
| ID | Runs | Label | Doc |
|---|---|---|---|
| align |
umi_alignment.cwl
(Workflow)
|
umi per-lane alignment subworkflow | |
| merge |
../tools/merge_bams.cwl
(CommandLineTool)
|
Sambamba: merge | |
| index_cram |
../tools/index_cram.cwl
(CommandLineTool)
|
samtools index cram | |
| bam_to_cram |
../tools/bam_to_cram.cwl
(CommandLineTool)
|
BAM to CRAM conversion | |
| clip_overlap |
../tools/clip_overlap.cwl
(CommandLineTool)
|
clip overlapping reads | |
| align_consensus |
../tools/realign.cwl
(CommandLineTool)
|
umi realignment | |
| filter_consensus |
../tools/filter_consensus.cwl
(CommandLineTool)
|
filter consensus reads | |
| group_reads_by_umi |
../tools/group_reads.cwl
(CommandLineTool)
|
group reads by umi | |
| call_molecular_consensus |
../tools/call_molecular_consensus.cwl
(CommandLineTool)
|
call molecular consensus | |
| collect_duplex_seq_metrics |
../tools/duplex_seq_metrics.cwl
(CommandLineTool)
|
collect duplex seq metrics |
Outputs
| ID | Type | Label | Doc |
|---|---|---|---|
| aligned_cram | File | ||
| adapter_histogram | File[] | ||
| duplex_seq_metrics | File[] |
Permalink:
https://w3id.org/cwl/view/git/fa0bf2a51b72cd0869253943b67aa8e271633945/definitions/subworkflows/molecular_alignment.cwl
