- Selected
- |
- Default Values
- Nested Workflows
- Tools
- Inputs/Outputs
This workflow is Open Source and may be reused according to the terms of:
MIT License
Note that the tools invoked by the workflow may have separate licenses.
Inputs
ID | Type | Title | Doc |
---|---|---|---|
sample_name | String | ||
QCannotation | File | ||
read_group_id | String[] | ||
reference_index | String | ||
reference_sizes | File | ||
trimming_adapters | File | ||
assay_non_cpg_sites |
Variable to determine if user wants to obtain obtain bed/bigwig files for non-CpG cytosines. Value - true or false |
||
instrument_data_bams | File[] | ||
trimming_max_uncalled | Integer | ||
trimming_min_readlength | Integer | ||
trimming_adapter_trim_end | String | ||
trimming_adapter_min_overlap | Integer |
Steps
ID | Runs | Label | Doc |
---|---|---|---|
merge |
../tools/merge_bams.cwl
(CommandLineTool)
|
Sambamba: merge | |
pileup |
../tools/biscuit_pileup.cwl
(CommandLineTool)
|
Biscuit pileup | |
vcf2bed |
../tools/bisulfite_vcf2bed.cwl
(CommandLineTool)
|
convert a biscuit pileup vcf to a bed and bedgraph file, via a wrapper script | |
index_cram |
../tools/index_cram.cwl
(CommandLineTool)
|
samtools index cram | |
bam_to_cram |
../tools/bam_to_cram.cwl
(CommandLineTool)
|
BAM to CRAM conversion | |
bisulfite_qc |
../subworkflows/bisulfite_qc.cwl
(Workflow)
|
Bisulfite QC tools | |
bedgraph_to_bigwig |
../tools/bedgraph_to_bigwig.cwl
(CommandLineTool)
|
bedGraph to bigwig conversion | |
bam_to_trimmed_fastq_and_biscuit_alignments | bam to trimmed fastqs and biscuit alignments |
Outputs
ID | Type | Label | Doc |
---|---|---|---|
vcf | File | ||
cpgs | File[] | ||
cram | File | ||
cpg_bigwig | File[] | ||
gathered_directory | Directory |
Permalink:
https://w3id.org/cwl/view/git/c625e05eefb1754353c1bdfa46c01dc61e6233dd/definitions/pipelines/bisulfite.cwl