Workflow: umi duplex alignment workflow

Fetched 2023-01-10 03:46:31 GMT
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Inputs

ID Type Title Doc
bam File[]
min_reads Integer[]
reference File
sample_name String
read_structure String[]
min_base_quality Integer
target_intervals File (Optional)
max_base_error_rate Float (Optional)
max_read_error_rate Float (Optional)
max_no_call_fraction Float

Steps

ID Runs Label Doc
align
umi_alignment.cwl (Workflow)
umi per-lane alignment subworkflow
merge
../tools/merge_bams.cwl (CommandLineTool)
Sambamba: merge
clip_overlap
../tools/clip_overlap.cwl (CommandLineTool)
clip overlapping reads
align_consensus
../tools/realign.cwl (CommandLineTool)
umi realignment
filter_consensus
../tools/filter_consensus.cwl (CommandLineTool)
filter consensus reads
group_reads_by_umi
../tools/group_reads.cwl (CommandLineTool)
group reads by umi
call_duplex_consensus
../tools/call_duplex_consensus.cwl (CommandLineTool)
call duplex consensus
collect_duplex_seq_metrics
../tools/duplex_seq_metrics.cwl (CommandLineTool)
collect duplex seq metrics

Outputs

ID Type Label Doc
aligned_bam File
adapter_histogram File[]
duplex_seq_metrics File[]
Permalink: https://w3id.org/cwl/view/git/7638b3075863ae8172f4adaec82fb2eb8e80d3d5/definitions/subworkflows/duplex_alignment.cwl