Workflow: Replace legacy AML Trio Assay
Fetched 2025-09-10 23:43:34 GMT
Verified with cwltool version 3.1.20230201224320
- Selected
- |
- Default Values
- Nested Workflows
- Tools
- Inputs/Outputs
This workflow is Open Source and may be reused according to the terms of:
MIT License
Note that the tools invoked by the workflow may have separate licenses.
Inputs
| ID | Type | Title | Doc |
|---|---|---|---|
| mills | File | ||
| docm_vcf | File | ||
| omni_vcf | File | ||
| vep_pick | |||
| dbsnp_vcf | File | ||
| intervals | 3b934bcd759f675a946c0ba749c30f12[] | ||
| reference | String | ||
| tumor_name | String (Optional) | ||
| normal_name | String (Optional) | ||
| known_indels | File | ||
| somalier_vcf | File | ||
| followup_name | String (Optional) | ||
| gvcf_gq_bands | String[] | ||
| interval_list | File | ||
| synonyms_file | File (Optional) | ||
| vep_cache_dir | String | ||
| bait_intervals | File | ||
| bqsr_intervals | String[] | ||
| cle_vcf_filter | Boolean | ||
| tumor_sequence | https://w3id.org/cwl/view/git/28d1065759cbd389594ee33b41fd1103ced5436d/definitions/types/sequence_data.yml#sequence_data[] | ||
| disclaimer_text | String (Optional) | ||
| normal_sequence | https://w3id.org/cwl/view/git/28d1065759cbd389594ee33b41fd1103ced5436d/definitions/types/sequence_data.yml#sequence_data[] | ||
| varscan_p_value | Float (Optional) | ||
| target_intervals | File | ||
| followup_sequence | https://w3id.org/cwl/view/git/28d1065759cbd389594ee33b41fd1103ced5436d/definitions/types/sequence_data.yml#sequence_data[] | ||
| summary_intervals | https://w3id.org/cwl/view/git/28d1065759cbd389594ee33b41fd1103ced5436d/definitions/types/labelled_file.yml#labelled_file[] | ||
| disclaimer_version | String | ||
| per_base_intervals | https://w3id.org/cwl/view/git/28d1065759cbd389594ee33b41fd1103ced5436d/definitions/types/labelled_file.yml#labelled_file[] | ||
| pindel_insert_size | Integer | ||
| pindel_region_file | File | ||
| germline_tsv_prefix | String (Optional) | ||
| vep_ensembl_species | String |
ensembl species - Must be present in the cache directory. Examples: homo_sapiens or mus_musculus |
|
| vep_ensembl_version | String |
ensembl version - Must be present in the cache directory. Example: 95 |
|
| vep_to_table_fields | String[] | ||
| annotate_coding_only | Boolean (Optional) | ||
| filter_docm_variants | Boolean (Optional) | ||
| filter_minimum_depth | Integer (Optional) | ||
| germline_coding_only | Boolean (Optional) | ||
| mutect_scatter_count | Integer | ||
| per_target_intervals | https://w3id.org/cwl/view/git/28d1065759cbd389594ee33b41fd1103ced5436d/definitions/types/labelled_file.yml#labelled_file[] | ||
| strelka_cpu_reserved | Integer (Optional) | ||
| varscan_min_coverage | Integer (Optional) | ||
| varscan_min_var_freq | Float (Optional) | ||
| vep_ensembl_assembly | String |
genome assembly to use in vep. Examples: GRCh38 or GRCm38 |
|
| varscan_strand_filter | Integer (Optional) | ||
| vep_custom_annotations | https://w3id.org/cwl/view/git/28d1065759cbd389594ee33b41fd1103ced5436d/definitions/types/vep_custom_annotation.yml#vep_custom_annotation[] |
custom type, check types directory for input format |
|
| qc_minimum_base_quality | Integer (Optional) | ||
| varscan_max_normal_freq | Float (Optional) | ||
| variants_to_table_fields | String[] | ||
| emit_reference_confidence | String | ||
| qc_minimum_mapping_quality | Integer (Optional) | ||
| variant_reporting_intervals | File | ||
| germline_vep_to_table_fields | String[] | ||
| picard_metric_accumulation_level | String | ||
| germline_variants_to_table_fields | String[] | ||
| variants_to_table_genotype_fields | String[] | ||
| germline_variants_to_table_genotype_fields | String[] |
Steps
| ID | Runs | Label | Doc |
|---|---|---|---|
| concordance |
../tools/concordance.cwl
(CommandLineTool)
|
Concordance checking between Tumor and Normal BAM | |
| pindel_region |
../subworkflows/pindel_region.cwl
(Workflow)
|
Run pindel on provided region | |
| extract_freemix |
aml_trio_cle.cwl#extract_freemix/2791e58a-05c8-4e0a-9fba-5c93a5663f9f
(ExpressionTool)
|
||
| tumor_index_cram |
../tools/index_cram.cwl
(CommandLineTool)
|
samtools index cram | |
| normal_index_cram |
../tools/index_cram.cwl
(CommandLineTool)
|
samtools index cram | |
| tumor_bam_to_cram |
../tools/bam_to_cram.cwl
(CommandLineTool)
|
BAM to CRAM conversion | |
| normal_bam_to_cram |
../tools/bam_to_cram.cwl
(CommandLineTool)
|
BAM to CRAM conversion | |
| followup_index_cram |
../tools/index_cram.cwl
(CommandLineTool)
|
samtools index cram | |
| full_variant_report |
../tools/cle_aml_trio_report_full_variants.cwl
(CommandLineTool)
|
cle aml_trio full variants report | |
| coverage_stat_report |
../tools/cle_aml_trio_report_coverage_stat.cwl
(CommandLineTool)
|
cle aml_trio hs_metrics coverage_stat report | |
| followup_bam_to_cram |
../tools/bam_to_cram.cwl
(CommandLineTool)
|
BAM to CRAM conversion | |
| alignment_stat_report |
../tools/cle_aml_trio_report_alignment_stat.cwl
(CommandLineTool)
|
cle aml_trio alignment_stat report | |
| tumor_detect_variants |
detect_variants.cwl
(Workflow)
|
Detect Variants workflow | |
| followup_bam_readcount |
../tools/bam_readcount.cwl
(CommandLineTool)
|
run bam-readcount | |
| tumor_alignment_and_qc |
alignment_exome.cwl
(Workflow)
|
exome alignment with qc | |
| normal_alignment_and_qc |
alignment_exome.cwl
(Workflow)
|
exome alignment with qc | |
| germline_detect_variants |
../subworkflows/germline_detect_variants.cwl
(Workflow)
|
exome alignment and germline variant detection | |
| followup_alignment_and_qc |
alignment_exome.cwl
(Workflow)
|
exome alignment with qc | |
| add_disclaimer_to_tumor_final_tsv |
../tools/add_string_at_line.cwl
(CommandLineTool)
|
Insert an arbitrary string at a specific line of a file | |
| add_disclaimer_to_germline_final_tsv |
../tools/add_string_at_line.cwl
(CommandLineTool)
|
Insert an arbitrary string at a specific line of a file | |
| add_disclaimer_to_full_variant_report |
../tools/add_string_at_line.cwl
(CommandLineTool)
|
Insert an arbitrary string at a specific line of a file | |
| add_disclaimer_version_to_tumor_final_tsv |
../tools/add_string_at_line.cwl
(CommandLineTool)
|
Insert an arbitrary string at a specific line of a file | |
| add_disclaimer_version_to_germline_final_tsv |
../tools/add_string_at_line.cwl
(CommandLineTool)
|
Insert an arbitrary string at a specific line of a file | |
| add_disclaimer_version_to_full_variant_report |
../tools/add_string_at_line.cwl
(CommandLineTool)
|
Insert an arbitrary string at a specific line of a file |
Outputs
| ID | Type | Label | Doc |
|---|---|---|---|
| tumor_cram | File | ||
| normal_cram | File | ||
| followup_cram | File | ||
| tumor_final_tsv | File | ||
| tumor_final_vcf | File | ||
| tumor_flagstats | File | ||
| normal_flagstats | File | ||
| tumor_hs_metrics | File | ||
| docm_filtered_vcf | File | ||
| normal_hs_metrics | File | ||
| pindel_region_vcf | File | ||
| tumor_vep_summary | File | ||
| followup_flagstats | File | ||
| germline_final_tsv | File | ||
| germline_final_vcf | File | ||
| followup_hs_metrics | File | ||
| full_variant_report | File | ||
| mutect_filtered_vcf | File | ||
| pindel_filtered_vcf | File | ||
| coverage_stat_report | File | ||
| strelka_filtered_vcf | File | ||
| varscan_filtered_vcf | File | ||
| alignment_stat_report | File | ||
| germline_filtered_tsv | File | ||
| germline_filtered_vcf | File | ||
| mutect_unfiltered_vcf | File | ||
| pindel_unfiltered_vcf | File | ||
| strelka_unfiltered_vcf | File | ||
| varscan_unfiltered_vcf | File | ||
| tumor_final_filtered_vcf | File | ||
| tumor_summary_hs_metrics | File[] | ||
| normal_summary_hs_metrics | File[] | ||
| tumor_insert_size_metrics | File | ||
| tumor_verify_bam_id_depth | File | ||
| normal_insert_size_metrics | File | ||
| normal_verify_bam_id_depth | File | ||
| followup_summary_hs_metrics | File[] | ||
| tumor_snv_bam_readcount_tsv | File | ||
| tumor_verify_bam_id_metrics | File | ||
| followup_insert_size_metrics | File | ||
| followup_verify_bam_id_depth | File | ||
| normal_snv_bam_readcount_tsv | File | ||
| normal_verify_bam_id_metrics | File | ||
| somalier_concordance_metrics | File | ||
| tumor_indel_bam_readcount_tsv | File | ||
| tumor_mark_duplicates_metrics | File | ||
| followup_snv_bam_readcount_tsv | File | ||
| followup_verify_bam_id_metrics | File | ||
| normal_indel_bam_readcount_tsv | File | ||
| normal_mark_duplicates_metrics | File | ||
| somalier_concordance_statistics | File | ||
| tumor_alignment_summary_metrics | File | ||
| followup_indel_bam_readcount_tsv | File | ||
| followup_mark_duplicates_metrics | File | ||
| normal_alignment_summary_metrics | File | ||
| followup_alignment_summary_metrics | File |
Permalink:
https://w3id.org/cwl/view/git/28d1065759cbd389594ee33b41fd1103ced5436d/definitions/pipelines/aml_trio_cle.cwl
