mills provides known polymorphic indels recommended by GATK for a variety of tools including the BaseRecalibrator. This file is part of the GATK resource bundle available at http://www.broadinstitute.org/gatk/guide/article?id=1213 Essentially it is a list of known indels originally discovered by mill et al. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1557762/ File should be in vcf format, and tabix indexed.

dbsnp_vcf provides known indels reecommended by GATK for a variety of tools including the BaseRecalibrator. This file is part of the GATK resource bundle available at http://www.broadinstitute.org/gatk/guide/article?id=1213 Essintially it is a list of known indels from dbSNP. File should be in vcf format, and tabix indexed.

reference contains the nucleotide sequence for a given assembly (hg37, hg38, etc.) in fasta format for the entire genome. This is what reads will be aligned to. Appropriate files can be found on ensembl at https://ensembl.org/info/data/ftp/index.html When providing the reference secondary files corresponding to reference indices must be located in the same directory as the reference itself. These files can be created with samtools index, bwa index, and picard CreateSequenceDictionary.

tumor_name provides a string for what the MT sample will be referred to in the various outputs, for exmaple the VCF files.

netmhc_stab sets an option that decides whether it will run NetMHCStabPan after all filtering and add stability predictions to predicted epitopes.

normal_name provides a string for what the WT sample will be referred to in the various outputs, for exmaple the VCF files.

known_indels provides known indels reecommended by GATK for a variety of tools including the BaseRecalibrator. This file is part of the GATK resource bundle available at http://www.broadinstitute.org/gatk/guide/article?id=1213 Essintially it is a list of known indels from 1000 Genomes Phase I indel calls. File should be in vcf format, and tabix indexed.

bait_intervals is an interval_list corresponding to the baits used in sequencing reagent. These are essentially coordinates for regions you were able to design probes for in the reagent. Typically the reagent provider has this information available in bed format and it can be converted to an interval_list with Picard BedToIntervalList. AstraZeneca also maintains a repo of baits for common sequencing reagents available at https://github.com/AstraZeneca-NGS/reference_data

bqsr_intervals provides an array of genomic intervals for which to apply GATK base quality score recalibrations. Typically intervals are given for the entire chromosome (i.e. chr1, chr2, etc.), these names should match the format in the reference file.

Previously discovered variants to be flagged in this pipelines's output vcf

tumor_sequence is a data structure described in sequence_data.yml used to pass information regarding sequencing data for single sample (i.e. fastq files). If more than one fastq file exist for a sample, as in the case for multiple instrument data, the sequence tag is simply repeated with the additional data (see example input file). Note that in the @RG field ID and SM are required.

net_chop_method is used to specify which NetChop prediction method to use (\"cterm\" for C term 3.0, \"20s\" for 20S 3.0). C-term 3.0 is trained with publicly available MHC class I ligands and the authors believe that is performs best in predicting the boundaries of CTL epitopes. 20S is trained with in vitro degradation data.

normal_sequence is a data structure described in sequence_data.yml used to pass information regarding sequencing data for single sample (i.e. fastq files). If more than one fastq file exist for a sample, as in the case for multiple instrument data, the sequence tag is simply repeated with the additional data (see example input file). Note that in the @RG field ID and SM are required.

pvacseq_threads specifies the number of threads to use for parallelizing peptide-MHC binding prediction calls.

target_intervals is an interval_list corresponding to the targets for the capture reagent. BED files with this information can be converted to interval_lists with Picard BedToIntervalList. In general for a WES exome reagent bait_intervals and target_intervals are the same.

tumor_sample_name is the name of the tumor sample being processed. When processing a multi-sample VCF the sample name must be a sample ID in the input VCF #CHROM header line.

net_chop_threshold specifies the threshold to use for NetChop prediction; increasing the threshold results in better specificity, but worse sensitivity.

normal_sample_name is the name of the normal sample to use for phasing of germline variants.

ensembl species - Must be present in the cache directory. Examples: homo_sapiens or mus_musculus

ensembl version - Must be present in the cache directory. Example: 95

genome assembly to use in vep. Examples: GRCh38 or GRCm38

custom type, check types directory for input format

The effective coverage of capture products generally extends out beyond the actual regions targeted. This parameter allows variants to be called in these wingspan regions, extending this many base pairs from each side of the target regions.

used to provide clinical HLA typing results in the format HLA-X*01:02[/HLA-X...] when available.

used to provide clinical HLA typing results; separated from class I due to nomenclature inconsistencies