Workflow: preprocess-ont.cwl

Fetched 2024-05-19 13:03:19 GMT
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Inputs

ID Type Title Doc
ref_human_genome File
illumina_accession String

Steps

ID Runs Label Doc
fastqc
../bio-cwl-tools/fastqc/fastqc_2.cwl (CommandLineTool)

Tool runs FastQC from Babraham Bioinformatics

multiqc
../bio-cwl-tools/multiqc/multiqc.cwl (CommandLineTool)

Run multiqc on log files from supported bioinformatic tools. 26/04/2020: Fixed output filenames, added support for a single input file in qc_files_array (Miguel Boland)

sam2bam
../bio-cwl-tools/samtools/samtools_view_sam2bam.cwl (CommandLineTool)

Convert SAM to BAM.

minimap2
../bio-cwl-tools/minimap2/minimap2.cwl (CommandLineTool)

This CWL file defines running minimap2 to align some sequences to a database. We assume the database has been indexed. This is not necessary but we will do it in our use case

nanoplot
../bio-cwl-tools/nanoplot/nanoplot.cwl (CommandLineTool)
rename_bam
../bio-cwl-tools/util/rename.cwl (ExpressionTool)

Also consider https://www.commonwl.org/user_guide/misc/#rename-an-input-file or https://www.commonwl.org/user_guide/misc/#rename-an-output-file

fetch_fastqs

Worfklow combining an SRA fetch from NCBI with a fastq-dump cmd

rename_fastq
../bio-cwl-tools/util/rename.cwl (ExpressionTool)

Also consider https://www.commonwl.org/user_guide/misc/#rename-an-input-file or https://www.commonwl.org/user_guide/misc/#rename-an-output-file

samtools_fastq
../bio-cwl-tools/samtools/samtools_fastq.cwl (CommandLineTool)

Bam to fastq conversion

rename_fastqc_zip
../bio-cwl-tools/util/rename.cwl (ExpressionTool)

Also consider https://www.commonwl.org/user_guide/misc/#rename-an-input-file or https://www.commonwl.org/user_guide/misc/#rename-an-output-file

rename_multiqc_zip
../bio-cwl-tools/util/rename.cwl (ExpressionTool)

Also consider https://www.commonwl.org/user_guide/misc/#rename-an-input-file or https://www.commonwl.org/user_guide/misc/#rename-an-output-file

rename_multiqc_html
../bio-cwl-tools/util/rename.cwl (ExpressionTool)

Also consider https://www.commonwl.org/user_guide/misc/#rename-an-input-file or https://www.commonwl.org/user_guide/misc/#rename-an-output-file

rename_fastqc_summary
../bio-cwl-tools/util/rename.cwl (ExpressionTool)

Also consider https://www.commonwl.org/user_guide/misc/#rename-an-input-file or https://www.commonwl.org/user_guide/misc/#rename-an-output-file

Outputs

ID Type Label Doc
bam File
fastqc_zip File
multiqc_zip File
multiqc_html File
fastqc_summary File
original_fastq1 File
Permalink: https://w3id.org/cwl/view/git/3114ddd28bbee5505a9819b1a84f4896fd0b0992/PreProcessing/preprocess-ont.cwl