Workflow: preprocess-illumina.cwl

Fetched 2024-11-23 20:13:01 GMT
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Inputs

ID Type Title Doc
hg38 File
illumina_accession String

Steps

ID Runs Label Doc
fastp
../bio-cwl-tools/fastp/fastp.cwl (CommandLineTool)

Modified from https://github.com/nigyta/bact_genome/blob/master/cwl/tool/fastp/fastp.cwl

bwa_mem
../bio-cwl-tools/bwa/BWA-Mem.cwl (CommandLineTool)
multiqc
../bio-cwl-tools/multiqc/multiqc.cwl (CommandLineTool)

Run multiqc on log files from supported bioinformatic tools. 26/04/2020: Fixed output filenames, added support for a single input file in qc_files_array (Miguel Boland)

rename_bam
../bio-cwl-tools/util/rename.cwl (ExpressionTool)

Also consider https://www.commonwl.org/user_guide/misc/#rename-an-input-file or https://www.commonwl.org/user_guide/misc/#rename-an-output-file

fetch_fastqs

Worfklow combining an SRA fetch from NCBI with a fastq-dump cmd

samtools_view
../bio-cwl-tools/samtools/samtools_view_sam2bam.cwl (CommandLineTool)

Convert SAM to BAM.

samtools_fastq
../bio-cwl-tools/samtools/samtools_fastq.cwl (CommandLineTool)

Bam to fastq conversion

rename_fastp_html
../bio-cwl-tools/util/rename.cwl (ExpressionTool)

Also consider https://www.commonwl.org/user_guide/misc/#rename-an-input-file or https://www.commonwl.org/user_guide/misc/#rename-an-output-file

rename_fastp_json
../bio-cwl-tools/util/rename.cwl (ExpressionTool)

Also consider https://www.commonwl.org/user_guide/misc/#rename-an-input-file or https://www.commonwl.org/user_guide/misc/#rename-an-output-file

unpack_ref_genome
tools/unpack_ref_db.cwl (CommandLineTool)

decompression tool using Lempel-Ziv coding (LZ77)

rename_multiqc_zip
../bio-cwl-tools/util/rename.cwl (ExpressionTool)

Also consider https://www.commonwl.org/user_guide/misc/#rename-an-input-file or https://www.commonwl.org/user_guide/misc/#rename-an-output-file

rename_multiqc_html
../bio-cwl-tools/util/rename.cwl (ExpressionTool)

Also consider https://www.commonwl.org/user_guide/misc/#rename-an-input-file or https://www.commonwl.org/user_guide/misc/#rename-an-output-file

Outputs

ID Type Label Doc
bam File [BAM]
multiqc_zip File [JSON]
mapped_fastq File [FASTQ]
multiqc_html File [HTML]
original_fastq1 File [FASTQ]
original_fastq2 File (Optional) [FASTQ]
fastp_html_report File [HTML]
fastp_json_report File [JSON]
Permalink: https://w3id.org/cwl/view/git/3114ddd28bbee5505a9819b1a84f4896fd0b0992/PreProcessing/preprocess-illumina.cwl