Workflow: Single-Cell Preprocessing Cell Ranger Pipeline
Devel version of Single-Cell Preprocessing Cell Ranger Pipeline ===============================================================
- Selected
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- Default Values
- Nested Workflows
- Tools
- Inputs/Outputs
Inputs
ID | Type | Title | Doc |
---|---|---|---|
alias | String | Experiment short name/Alias | |
threads | Integer (Optional) | Number of threads |
Number of threads for those steps that support multithreading |
memory_limit | Integer (Optional) | Genome Type |
Maximum memory used (GB). The same as was used for generating indices. The same will be applied to virtual memory |
fastq_file_r1 | File [FASTQ] | FASTQ file R1 (optionally compressed) |
FASTQ file R1 (optionally compressed) |
fastq_file_r2 | File [FASTQ] | FASTQ file R2 (optionally compressed) |
FASTQ file R2 (optionally compressed) |
indices_folder | Directory | Genome Type |
Cell Ranger generated genome indices folder |
Steps
ID | Runs | Label | Doc |
---|---|---|---|
extract_fastq_r1 |
../tools/extract-fastq.cwl
(CommandLineTool)
|
Tool to decompress input FASTQ file(s). If several FASTQ files are provided, they will be concatenated in the order that corresponds to files in input. Bash script's logic: - disable case sensitive glob check - check if root name of input file already include '.fastq' or '.fq' extension. If yes, set DEFAULT_EXT to \"\", otherwise use '.fastq' - check file type, decompress if needed - return 1, if file type is not recognized This script also works of input file doesn't have any extension at all |
|
extract_fastq_r2 |
../tools/extract-fastq.cwl
(CommandLineTool)
|
Tool to decompress input FASTQ file(s). If several FASTQ files are provided, they will be concatenated in the order that corresponds to files in input. Bash script's logic: - disable case sensitive glob check - check if root name of input file already include '.fastq' or '.fq' extension. If yes, set DEFAULT_EXT to \"\", otherwise use '.fastq' - check file type, decompress if needed - return 1, if file type is not recognized This script also works of input file doesn't have any extension at all |
|
collect_statistics |
single-cell-preprocess-cellranger.cwl#collect_statistics/66247ebc-cae6-40ab-9193-85e3a8632970
(CommandLineTool)
|
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estimate_contamination |
../tools/soupx-subworkflow.cwl
(Workflow)
|
SoupX (workflow) - an R package for the estimation and removal of cell free mRNA contamination |
Wrapped in a workflow SoupX tool for easy access to Cell Ranger pipeline compressed outputs. |
generate_counts_matrix |
../tools/cellranger-count.cwl
(CommandLineTool)
|
Cellranger count - generates single cell feature counts for a single library |
Generates single cell feature counts for a single library. |
run_fastqc_for_fastq_r1 |
../tools/fastqc.cwl
(CommandLineTool)
|
Tool runs FastQC from Babraham Bioinformatics |
|
run_fastqc_for_fastq_r2 |
../tools/fastqc.cwl
(CommandLineTool)
|
Tool runs FastQC from Babraham Bioinformatics |
|
compress_raw_feature_bc_matrices_folder |
../tools/tar-compress.cwl
(CommandLineTool)
|
Compresses input directory to tar.gz |
|
compress_secondary_analysis_report_folder |
../tools/tar-compress.cwl
(CommandLineTool)
|
Compresses input directory to tar.gz |
|
compress_filtered_feature_bc_matrix_folder |
../tools/tar-compress.cwl
(CommandLineTool)
|
Compresses input directory to tar.gz |
Outputs
ID | Type | Label | Doc |
---|---|---|---|
molecule_info_h5 | File | Molecule-level information for aggregating samples into larger datasets |
Molecule-level information used by cellranger aggr to aggregate samples into larger datasets |
web_summary_report | File | Run summary metrics and charts in HTML format |
Run summary metrics and charts in HTML format |
loupe_browser_track | File | Loupe Browser visualization and analysis file |
Loupe Browser visualization and analysis file |
collected_statistics | File | Collected statistics in Markdown format |
Collected statistics in Markdown format |
fastqc_report_fastq_r1 | File | FastqQC report for FASTQ file R1 |
FastqQC report for FASTQ file R1 |
fastqc_report_fastq_r2 | File | FastqQC report for FASTQ file R2 |
FastqQC report for FASTQ file R2 |
metrics_summary_report | File | Run summary metrics in CSV format |
Run summary metrics in CSV format |
possorted_genome_bam_bai | File | Aligned to the genome indexed reads BAM+BAI files |
Indexed reads aligned to the genome and transcriptome annotated with barcode information |
raw_feature_bc_matrices_h5 | File | Unfiltered feature-barcode matrices in HDF5 format |
Unfiltered feature-barcode matrices containing all barcodes in HDF5 format |
contamination_estimation_plot | File | SoupX contamination estimation plot |
SoupX contamination estimation plot |
filtered_feature_bc_matrix_h5 | File | Filtered feature-barcode matrices in HDF5 format |
Filtered feature-barcode matrices containing only cellular barcodes in HDF5 format. When implemented, in Targeted Gene Expression samples, the non-targeted genes won't be present. |
raw_feature_bc_matrices_folder | File | Compressed folder with unfiltered feature-barcode matrices |
Compressed folder with unfiltered feature-barcode matrices containing all barcodes in MEX format |
adjusted_feature_bc_matrices_h5 | File | SoupX adjusted feature-barcode matrices in HDF5 format |
SoupX adjusted feature-barcode matrices in HDF5 format |
secondary_analysis_report_folder | File | Compressed folder with secondary analysis results |
Compressed folder with secondary analysis results including dimensionality reduction, cell clustering, and differential expression |
filtered_feature_bc_matrix_folder | File | Compressed folder with filtered feature-barcode matrices |
Compressed folder with filtered feature-barcode matrices containing only cellular barcodes in MEX format. When implemented, in Targeted Gene Expression samples, the non-targeted genes won't be present. |
generate_counts_matrix_stderr_log | File | stderr log generated by cellranger count |
stderr log generated by cellranger count |
generate_counts_matrix_stdout_log | File | stdout log generated by cellranger count |
stdout log generated by cellranger count |
adjusted_feature_bc_matrices_folder | File | Compressed folder with SoupX adjusted feature-barcode matrices |
Compressed folder with SoupX adjusted feature-barcode matrices in MEX format |
https://w3id.org/cwl/view/git/a1f6ca50fcb0881781b3ba0306dd61ebf555eaba/workflows/single-cell-preprocess-cellranger.cwl