Workflow: AltAnalyze Iterative Clustering and Guide-gene Selection

Fetched 2023-04-08 04:48:19 GMT

Devel version of AltAnalyze Iterative Clustering and Guide-gene Selection =========================================================================

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Inputs

ID Type Title Doc
alias String Experiment short name/Alias
downsample Integer (Optional) Downsample to

Downsample to

genome_data Directory AltAnalyze Prepare Genome Experiment

Ensembl database from the altanalyze-prepare-genome.cwl pipeline

restrict_by Restrict by

Restrict by

remove_outliers Boolean (Optional) Remove outliers

Remove outliers

exclude_cell_cycle Boolean (Optional) Exclude cell cycle

Exclude cell cycle

marker_pearson_cutoff Float (Optional) Marker Pearson cutoff

Marker Pearson cutoff

raw_feature_bc_matrices_h5 File scRNA-Seq Cell Ranger Experiment

Unfiltered feature-barcode matrices in HDF5 format from cellranger count or aggr results

Steps

ID Runs Label Doc
altanalyze_icgs
../tools/altanalyze-icgs.cwl (CommandLineTool)

Runs AltAnalyze Iterative Clustering and Guide-gene Selection for 10X Genomics data

cellbrowser_build
../tools/cellbrowser-build.cwl (CommandLineTool)

Converts AltAnalyze outputs into the data structure supported by UCSC CellBrowser

compress_html_data_folder
../tools/tar-compress.cwl (CommandLineTool)

Compresses input directory to tar.gz

compress_icgs_data_folder
../tools/tar-compress.cwl (CommandLineTool)

Compresses input directory to tar.gz

Outputs

ID Type Label Doc
html_data_folder Directory Folder with not compressed CellBrowser formatted results

Folder with not compressed CellBrowser formatted results

cellbrowser_report File CellBrowser formatted AltAnalyze ICGS report

CellBrowser formatted AltAnalyze ICGS report

altanalyze_icgs_stderr_log File stderr log generated by altanalyze icgs

stderr log generated by altanalyze icgs

altanalyze_icgs_stdout_log File stdout log generated by altanalyze icgs

stdout log generated by altanalyze icgs

compressed_html_data_folder File Compressed folder with CellBrowser formatted results

Compressed folder with CellBrowser formatted results

compressed_icgs_data_folder File Compressed folder with AltAnalyze ICGS results

Compressed folder with AltAnalyze ICGS results

Permalink: https://w3id.org/cwl/view/git/a1f6ca50fcb0881781b3ba0306dd61ebf555eaba/workflows/altanalyze-icgs.cwl