Workflow: SoupX Estimate

Fetched 2023-01-09 20:02:38 GMT

SoupX Estimate ==============

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Inputs

ID Type Title Doc
fdr Float (Optional) FDR cutoff for expression ratio plots

FDR cutoff for expression ratio plots

alias String Experiment short name/alias
round_counts Boolean (Optional) Round adjusted counts to integers

Round adjusted counts to integers

genelist_file File [Textual format] Target genes list. Headerless text file with 1 gene per line

Target genes list. Headerless text file with 1 gene per line

expression_threshold Float (Optional) Expression threshold for displaying target genes on a plot (expression > threshold)

Expression threshold for displaying target genes on a plot (expression > threshold)

raw_feature_bc_matrices_folder File [GZIP format] scRNA-Seq Cell Ranger Experiment

Compressed folder with unfiltered feature-barcode matrices

secondary_analysis_report_folder File [GZIP format] scRNA-Seq Cell Ranger Experiment

Compressed folder with secondary analysis results

filtered_feature_bc_matrix_folder File [GZIP format] scRNA-Seq Cell Ranger Experiment

Compressed folder with filtered feature-barcode matrices

Steps

ID Runs Label Doc
estimate_contamination SoupX (workflow) - an R package for the estimation and removal of cell free mRNA contamination

Wrapped in a workflow SoupX tool for easy access to Cell Ranger pipeline compressed outputs.

Outputs

ID Type Label Doc
soupx_stderr_log File [Textual format] SoupX stderr log

SoupX stderr log

soupx_stdout_log File [Textual format] SoupX stdout log

SoupX stdout log

raw_gene_expression_plots File (Optional) [PDF] Raw gene expression plots

Raw gene expression plots

contamination_estimation_plot File [PDF] Contamination estimation plot

Contamination estimation plot

adjusted_gene_expression_plots File (Optional) [PDF] Adjusted gene expression plots

Adjusted gene expression plots

adjusted_feature_bc_matrices_h5 File [HDF5] Adjusted feature-barcode matrices in HDF5 format

Adjusted feature-barcode matrices in HDF5 format

adjusted_feature_bc_matrices_folder File [GZIP format] Compressed folder with adjusted feature-barcode matrices in MEX format

Compressed folder with adjusted feature-barcode matrices in MEX format

raw_to_adjusted_gene_expression_ratio_plots File (Optional) [PDF]

Raw to adjusted gene expression ratio plots

raw_gene_expression_to_pure_soup_ratio_plots File (Optional) [PDF] Raw gene expression to pure soup ratio plots

Raw gene expression to pure soup ratio plots

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