- Selected
- |
- Default Values
- Nested Workflows
- Tools
- Inputs/Outputs
This workflow is Open Source and may be reused according to the terms of:
MIT License
Note that the tools invoked by the workflow may have separate licenses.
Inputs
ID | Type | Title | Doc |
---|---|---|---|
trimming | https://w3id.org/cwl/view/git/bfcb5ffbea3d00a38cc03595d41e53ea976d599d/definitions/types/trimming_options.yml#trimming_options (Optional) | ||
reference | File | ||
unaligned | https://w3id.org/cwl/view/git/bfcb5ffbea3d00a38cc03595d41e53ea976d599d/definitions/types/sequence_data.yml#sequence_data[] | ||
final_name | String | ||
bqsr_intervals | String[] (Optional) | ||
bqsr_known_sites | File[] |
One or more databases of known polymorphic sites used to exclude regions around known polymorphisms from analysis. |
Steps
ID | Runs | Label | Doc |
---|---|---|---|
bqsr |
../tools/bqsr.cwl
(CommandLineTool)
|
create BQSR table | |
align |
sequence_align_and_tag_adapter.cwl
(Workflow)
|
adapter for sequence_align_and_tag |
Some workflow engines won't stage files in our nested structure, so parse it out here |
merge |
../tools/merge_bams.cwl
(CommandLineTool)
|
Sambamba: merge | |
index_bam |
../tools/index_bam.cwl
(CommandLineTool)
|
samtools index | |
name_sort |
../tools/name_sort.cwl
(CommandLineTool)
|
sort BAM by name | |
apply_bqsr |
../tools/apply_bqsr.cwl
(CommandLineTool)
|
apply BQSR | |
mark_duplicates_and_sort |
../tools/mark_duplicates_and_sort.cwl
(CommandLineTool)
|
Mark duplicates and Sort |
Outputs
ID | Type | Label | Doc |
---|---|---|---|
final_bam | File | ||
mark_duplicates_metrics_file | File |
Permalink:
https://w3id.org/cwl/view/git/bfcb5ffbea3d00a38cc03595d41e53ea976d599d/definitions/subworkflows/sequence_to_bqsr.cwl