- Selected
- |
- Default Values
- Nested Workflows
- Tools
- Inputs/Outputs
Inputs
ID | Type | Title | Doc |
---|---|---|---|
nthreads_map | Integer |
Number of threads required for the 03-map step |
|
nthreads_quant | Integer |
Number of threads required for the 05-quantification step |
|
genome_sizes_file | File |
Genome sizes tab-delimited file (used in samtools) |
|
input_fastq_files | File[] | ||
nthreads_peakcall | Integer |
Number of threads required for the 04-peakcall step |
|
default_adapters_file | File |
Adapters file |
|
ENCODE_blacklist_bedfile | File |
Bedfile containing ENCODE consensus blacklist regions to be excluded. |
|
genome_ref_first_index_file | File |
\"First index file of Bowtie reference genome with extension 1.ebwt. \ (Note: the rest of the index files MUST be in the same folder)\" |
Steps
ID | Runs | Label | Doc |
---|---|---|---|
map |
01-map-se.cwl
(Workflow)
|
DNase-seq 01 mapping - reads: SE |
|
quant |
03-quantification.cwl
(Workflow)
|
DNase-seq 03 quantification |
|
peak_call |
02-peakcall.cwl
(Workflow)
|
DNase-seq 02 quantification |
Outputs
ID | Type | Label | Doc |
---|---|---|---|
map_pbc_files | File[] |
PCR Bottleneck Coefficient files (used to flag samples when pbc<0.5) |
|
map_read_count | File[] |
Read counts of the mapped BAM files |
|
fastq_read_count | File[] |
Read counts of the (unprocessed) input fastq files |
|
map_bowtie_log_files | File[] |
Bowtie log file with mapping stats |
|
map_filtered_bam_files | File[] |
Filtered BAM files (post-processing end point) |
|
quant_bigwig_raw_files | File[] |
Raw reads bigWig (signal) files |
|
map_filtered_read_count | File[] |
Read counts of the mapped and filtered BAM files |
|
peak_call_peak_xls_file | File[] |
Peak calling report file |
|
quant_bigwig_norm_files | File[] |
Normalized reads bigWig (signal) files |
|
map_preseq_c_curve_files | File[] |
Preseq c_curve output files |
|
map_percentage_uniq_reads | File[] |
Percentage of uniq reads from preseq c_curve output |
|
peak_call_narrowpeak_file | File[] |
Peaks in narrowPeak file format |
|
peak_call_spp_x_cross_corr | File[] |
SPP strand cross correlation summary |
|
quant_bigwig_extended_files | File[] |
Fragment extended reads bigWig (signal) files |
|
peak_call_spp_x_cross_corr_plot | File[] |
SPP strand cross correlation plot |
|
quant_bigwig_extended_norm_files | File[] |
Normalized fragment extended reads bigWig (signal) files |
|
peak_call_extended_narrowpeak_file | File[] |
Extended fragment peaks in narrowPeak file format |
|
peak_call_filtered_read_count_file | File[] |
Filtered read count after peak calling |
|
peak_call_peak_count_within_replicate | File[] |
Peak counts within replicate |
|
peak_call_read_in_peak_count_within_replicate | File[] |
Peak counts within replicate |
https://w3id.org/cwl/view/git/517487e59d240c197fc91f08d20dadca97a9e121/v1.0/DNase-seq_pipeline/pipeline-se.cwl