Workflow: pipeline-se.cwl

Fetched 2023-01-04 17:55:04 GMT

DNase-seq pipeline - reads: SE

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Inputs

ID Type Title Doc
nthreads_map Integer

Number of threads required for the 03-map step

nthreads_quant Integer

Number of threads required for the 05-quantification step

genome_sizes_file File

Genome sizes tab-delimited file (used in samtools)

input_fastq_files File[]
nthreads_peakcall Integer

Number of threads required for the 04-peakcall step

default_adapters_file File

Adapters file

ENCODE_blacklist_bedfile File

Bedfile containing ENCODE consensus blacklist regions to be excluded.

genome_ref_first_index_file File

\"First index file of Bowtie reference genome with extension 1.ebwt. \ (Note: the rest of the index files MUST be in the same folder)\"

Steps

ID Runs Label Doc
map
01-map-se.cwl (Workflow)

DNase-seq 01 mapping - reads: SE

quant

DNase-seq 03 quantification

peak_call
02-peakcall.cwl (Workflow)

DNase-seq 02 quantification

Outputs

ID Type Label Doc
map_pbc_files File[]

PCR Bottleneck Coefficient files (used to flag samples when pbc<0.5)

map_read_count File[]

Read counts of the mapped BAM files

fastq_read_count File[]

Read counts of the (unprocessed) input fastq files

map_bowtie_log_files File[]

Bowtie log file with mapping stats

map_filtered_bam_files File[]

Filtered BAM files (post-processing end point)

quant_bigwig_raw_files File[]

Raw reads bigWig (signal) files

map_filtered_read_count File[]

Read counts of the mapped and filtered BAM files

peak_call_peak_xls_file File[]

Peak calling report file

quant_bigwig_norm_files File[]

Normalized reads bigWig (signal) files

map_preseq_c_curve_files File[]

Preseq c_curve output files

map_percentage_uniq_reads File[]

Percentage of uniq reads from preseq c_curve output

peak_call_narrowpeak_file File[]

Peaks in narrowPeak file format

peak_call_spp_x_cross_corr File[]

SPP strand cross correlation summary

quant_bigwig_extended_files File[]

Fragment extended reads bigWig (signal) files

peak_call_spp_x_cross_corr_plot File[]

SPP strand cross correlation plot

quant_bigwig_extended_norm_files File[]

Normalized fragment extended reads bigWig (signal) files

peak_call_extended_narrowpeak_file File[]

Extended fragment peaks in narrowPeak file format

peak_call_filtered_read_count_file File[]

Filtered read count after peak calling

peak_call_peak_count_within_replicate File[]

Peak counts within replicate

peak_call_read_in_peak_count_within_replicate File[]

Peak counts within replicate

Permalink: https://w3id.org/cwl/view/git/517487e59d240c197fc91f08d20dadca97a9e121/v1.0/DNase-seq_pipeline/pipeline-se.cwl