Workflow: wf_get_reproducible_eclip_peaks.cwl

Fetched 2023-01-12 06:57:50 GMT

The main workflow that produces two reproducible peaks via IDR given two eCLIP samples (1 input, 1 IP each).

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Inputs

ID Type Title Doc
merged_peaks_bed String
rep1_clip_bam_file File
rep2_clip_bam_file File
rep1_input_bam_file File
rep1_peaks_bed_file File
rep2_input_bam_file File
rep2_peaks_bed_file File
merged_peaks_custombed String

Steps

ID Runs Label Doc
idr
idr.cwl (CommandLineTool)
create_bed_from_idr
parse_idr_peaks.cwl (CommandLineTool)

returns the

get_reproducing_peaks
get_reproducing_peaks.cwl (CommandLineTool)
count_reproducing_peaks
linescount.cwl (CommandLineTool)
rep1_input_norm_and_entropy

This workflow normalizes clip aligned reads against a size-matched input sample. Then, an entropy score is calculated for each peak found.

rep2_input_norm_and_entropy

This workflow normalizes clip aligned reads against a size-matched input sample. Then, an entropy score is calculated for each peak found.

reproducible_peaks_file_to_int
file2int.cwl (ExpressionTool)

Returns string expression based on file contents.

rep1_input_norm_using_idr_peaks
overlap_peakfi_with_bam.cwl (CommandLineTool)

This tool wraps Usage:

rep2_input_norm_using_idr_peaks
overlap_peakfi_with_bam.cwl (CommandLineTool)

This tool wraps Usage:

Outputs

ID Type Label Doc
idr_output File
idr_output_bed File
rep1_entropy_bed File
rep2_entropy_bed File
rep1_entropy_full File
rep2_entropy_full File
rep1_clip_read_num File
rep2_clip_read_num File
rep1_input_read_num File
rep2_input_read_num File
merged_peaks_bed_file File
rep1_input_normed_bed File
rep2_input_normed_bed File
rep1_input_normed_full File
rep2_input_normed_full File
reproducing_peaks_count Integer
rep1_entropy_excess_reads File
rep2_entropy_excess_reads File
merged_peaks_custombed_file File
rep1_reproducing_peaks_full File
rep2_reproducing_peaks_full File
rep1_idr_output_input_normed_bed File
rep2_idr_output_input_normed_bed File
rep1_idr_output_input_normed_full File
rep2_idr_output_input_normed_full File
Permalink: https://w3id.org/cwl/view/git/b922446e59a5e8afbc75e88d09567e256cefbbfd/cwl/wf_get_reproducible_eclip_peaks.cwl