Workflow: fasta2taxa-plot

Fetched 2024-11-23 21:54:37 GMT

Input is a fasta file with n>1 samples, with sample id as sequence identifier prefix, and a sample id file. The workflow calls open otus and assigns taxa using greengenes. The output are taxa plots.

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Inputs

ID Type Title Doc
reads
sample-mapping File
min-observations-per-otu Integer (Optional)
min-observations-per-sample Integer (Optional)
barcode-is-reverse-complement Boolean (Optional)
summarize-for-taxonomic-levels

Steps

ID Runs Label Doc
cluster
../../Tools/qiime/pick_de_novo_otus.cwl (CommandLineTool)
taxonomy
../../Tools/qiime/assign_taxonomy.cwl (CommandLineTool)
otu-table
../../Tools/qiime/make_otu_table.cwl (CommandLineTool)
join-reads
../../Tools/qiime/join_paired_ends.cwl (CommandLineTool)
sort-table
../../Tools/qiime/sort_otu_table.cwl (CommandLineTool)
filter-otus
../../Tools/qiime/filter_otus_from_otu_table.cwl (CommandLineTool)
beta-diversity
../../Tools/qiime/beta_diversity_through_plots.cwl (CommandLineTool)
filter-samples
../../Tools/qiime/filter_samples_from_otu_table.cwl (CommandLineTool)
summarize-taxa
../../Tools/qiime/summarize_taxa.cwl (CommandLineTool)
alpha-diversity
../../Tools/qiime/alpha_diversity.cwl (CommandLineTool)
samples-to-fasta
../../Tools/qiime/split_libraries_fastq.cwl (CommandLineTool)
validate-mapping
../../Tools/qiime/validate_mapping_file.cwl (CommandLineTool)
plot-taxa-summary
../../Tools/qiime/plot_taxa_summary.cwl (CommandLineTool)

Outputs

ID Type Label Doc
results Directory[]
error-logs File[]

Error logs for each step, collected by default and can be empty.

split-libraries Directory

output of split_libraries_fastq.py, demultiplex and quality filter (at Phred >= Q20)

taxa-summary-plots Directory
beta-diversity-results Directory
validated-mapping-file File

mapping file validated with validate_mapping_file.py

aplha-diversity-results File
Permalink: https://w3id.org/cwl/view/git/962607ff14f4468ef8114b76c6e8c1ed5e543e3f/CWL/Workflows/qiime/join-reads2plot.cwl