Workflow: metabarcode (gene amplicon) analysis for fastq files

Fetched 2023-01-08 22:12:16 GMT

protein - qc, preprocess, annotation, index, abundance

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Inputs

ID Type Title Doc
jobid String
m5nrBDB File
m5nrSCG File
filterLn Boolean
m5nrFull File[]
maxAmbig Integer
deviation Float
sequences File
filterAmbig Boolean

Steps

ID Runs Label Doc
qcBasic
annotate protein annotation

Proteins - predict, cluster, identify, annotate

abundance abundance

abundace profiles from annotated files, for protein and/or rna

preProcess preprocess fasta

Remove reads from fasta files based on sequence stats. Return fasta files with reads passed and reads removed.

indexSimSeq index sim seq

create sorted / filtered similarity file with feature sequences, and index by md5

Outputs

ID Type Label Doc
qcStatOut File
seqBinOut File
simSeqOut File
seqStatOut File
protSimsOut File
qcSummaryOut File
adapterPassed File
lcaProfileOut File
md5ProfileOut File
protFeatureOut File
sourceStatsOut File
protClustMapOut File
protClustSeqOut File
preProcessPassed File
preProcessRemoved File
Permalink: https://w3id.org/cwl/view/git/81feefc84ec0faecf1ade718001d5f07610e616e/CWL/Workflows/metabarcode-fasta.workflow.cwl