Workflow: TAP

Fetched 2023-01-11 20:29:01 GMT
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Inputs

ID Type Title Doc
primer

Euk and Prokaryote primer

tidy_up Boolean
indexDir Directory

Directory containing bowtie indices. Must containe index files with 'genome' prefix.

mate_pair

List of forward and reverse compressed fastq file records

ion_torrent Boolean
reference_database File [null]

Reference database, e.g. UNITE or SILVA

reference_taxonomy File

Taxonomy mapping from accession to tax string

Steps

ID Runs Label Doc
PHIX
../Tools/bowtie2.tool.cwl (CommandLineTool)
PHIX

STAGE:0060 PHIX removal using bowtie2 with Illumina RTA genome and Illumina built indeces

prep
../Tools/cutadapt.tool.cwl (CommandLineTool)
cutadapt

none

cluster
../Tools/vsearch/Clustering.vsearch.cwl (CommandLineTool)
Clustering

Clustering

merging
../Tools/vsearch.tool.cwl (CommandLineTool)
vsearch

vsearcg

cleanReads
../Tools/vsearch/Searching.vsearch.cwl (CommandLineTool)
Searching

Searching

decompress Decompress

Decompress mate pair fastq files

dereplicate
../Tools/vsearch/Dereplication_and_rereplication.vsearch.cwl (CommandLineTool)
Dereplication_and_rereplication

Dereplication and rereplication

convertToOTU
../Tools/uc2otu.tool.cwl (CommandLineTool)
uc2otu

n/a

removePrimer
../Tools/cutadapt.tool.cwl (CommandLineTool)
cutadapt

none

classification
../Tools/mothur/classification.mothur.tool.cwl (CommandLineTool)
mothur command line

n/a

extractFeatures
../Tools/metaxa2_x.tool.cwl (CommandLineTool)
metaxa2_x

n/a

removeCommentsAddBarcodeLabel
../Tools/regexp.tool.cwl (CommandLineTool)
sed

n/a

Outputs

ID Type Label Doc
raw https://w3id.org/cwl/salad#Any
tmp File
OTUs File
merged File
noPHIX File
features File[]
noPrimer File
clustered File
Classified File[]
RegexpTool File[]
cleanedReads File[]
dereplicated File
Permalink: https://w3id.org/cwl/view/git/67fc58a072d6a72658a54244f6dded172f57314c/CWL/Workflows/tap.prok.workflow.cwl