Workflow: seqprep-subwf.cwl

Fetched 2024-05-09 10:14:42 GMT
children parents
Workflow as SVG
  • Selected
  • Default Values
  • Nested Workflows
  • Tools
  • Inputs/Outputs

Inputs

ID Type Title Doc
single_reads File (Optional)
forward_reads File (Optional)
reverse_reads File (Optional)
paired_reads_length_filter Integer

Steps

ID Runs Label Doc
filter_paired
../../utils/fastp.cwl (CommandLineTool)

Modified from https://github.com/nigyta/bact_genome/blob/master/cwl/tool/fastp/fastp.cwl

overlap_reads
../../tools/SeqPrep/seqprep.cwl (CommandLineTool)
Tool for stripping adaptors and/or merging paired reads with overlap into single reads.

SeqPrep is a program to merge paired end Illumina reads that are overlapping into a single longer read. It may also just be used for its adapter trimming feature without doing any paired end overlap. When an adapter sequence is present, that means that the two reads must overlap (in most cases) so they are forcefully merged. When reads do not have adapter sequence they must be treated with care when doing the merging, so a much more specific approach is taken. The default parameters were chosen with specificity in mind, so that they could be ran on libraries where very few reads are expected to overlap. It is always safest though to save the overlapping procedure for libraries where you have some prior knowledge that a significant portion of the reads will have some overlap.

unzip_merged_reads
../../utils/multiple-gunzip.cwl (CommandLineTool)
unzip files
unzip_single_reads
../../utils/multiple-gunzip.cwl (CommandLineTool)
unzip files
count_submitted_reads
../../utils/count_lines/count_lines.cwl (CommandLineTool)
count_submitted_reads_single
../../utils/count_lines/count_lines.cwl (CommandLineTool)

Outputs

ID Type Label Doc
fastp_report File (Optional)
unzipped_single_reads File
count_forward_submitted_reads Integer
Permalink: https://w3id.org/cwl/view/git/a83ee883bb3c7480010fa952939fac771491ddf4/workflows/subworkflows/seqprep-subwf.cwl