Workflow: exomeseq-gatk4-01-preprocessing.cwl
Fetched 2025-05-02 06:47:09 GMT
Verified with cwltool version 3.1.20230201224320
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- Inputs/Outputs
This workflow is Open Source and may be reused according to the terms of:
MIT License
Note that the tools invoked by the workflow may have separate licenses.
Inputs
| ID | Type | Title | Doc |
|---|---|---|---|
| library | String | ||
| threads | Integer | ||
| platform | String | ||
| intervals | File[] (Optional) | ||
| read_pair | https://w3id.org/cwl/view/git/cb2c1423d635b2d8527103835b4918ffdf1f5b80/types/bespin-types.yml#FASTQReadPairType | ||
| known_sites | File[] | ||
| reference_genome | File | ||
| bait_interval_list | File | ||
| target_interval_list | File |
Steps
| ID | Runs | Label | Doc |
|---|---|---|---|
| qc |
../tools/fastqc.cwl
(CommandLineTool)
|
||
| map |
../tools/gitc-bwa-mem-samtools.cwl
(CommandLineTool)
|
||
| sort |
../tools/GATK4-SortSam.cwl
(CommandLineTool)
|
||
| trim |
../tools/trim_galore.cwl
(CommandLineTool)
|
||
| fixtags |
../tools/GATK4-SetNmAndUqTags.cwl
(CommandLineTool)
|
||
| combine_reads |
../tools/concat-gz-files.cwl
(CommandLineTool)
|
||
| mark_duplicates |
../tools/GATK4-MarkDuplicates.cwl
(CommandLineTool)
|
||
| file_pair_details |
../tools/extract-named-file-pair-details.cwl
(ExpressionTool)
|
Given a FASTQReadPairType returns a 2D array of the files contained within | |
| recalibrate_01_analyze |
../tools/GATK4-BaseRecalibrator.cwl
(CommandLineTool)
|
||
| generate_sample_filenames |
../tools/generate-sample-filenames.cwl
(ExpressionTool)
|
Generates a set of file names for preprocessing steps based on an input sample name | |
| recalibrate_02_apply_bqsr |
../tools/GATK4-ApplyBQSR.cwl
(CommandLineTool)
|
Outputs
| ID | Type | Label | Doc |
|---|---|---|---|
| trim_reports | File[] | ||
| fastqc_reports | File[] | ||
| markduplicates_bam | File | ||
| recalibrated_reads | File | ||
| recalibration_table | File |
Permalink:
https://w3id.org/cwl/view/git/cb2c1423d635b2d8527103835b4918ffdf1f5b80/subworkflows/exomeseq-gatk4-01-preprocessing.cwl
