Workflow: ATACseq_pipeline.cwl

Fetched 2023-01-13 06:47:05 GMT
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Inputs

ID Type Title Doc
fastq1 File[]
fastq2 File[]
adapter1 String[]
adapter2 String[]
bin_size Integer
reference File
sample_id String
macs2_qvalue Float
reference_info File
macs2_genome_size String
effective_genome_size Long
ignoreForNormalization String[]
max_mapping_insert_length Long

Steps

ID Runs Label Doc
trim_and_map
merge_duprem_filter
qc_plot_fingerprint
../tools/deeptools_plotFingerprint.cwl (CommandLineTool)
converting_bam_to_bedpe
../tools/bedtools_bamtobed_pe.cwl (CommandLineTool)
qc_phantompeakqualtools
../tools/phantompeakqualtools.cwl (CommandLineTool)
create_summary_qc_report
../tools/multiqc_hack.cwl (CommandLineTool)
peak_calling_macs2_broad
../tools/macs2_callpeak_atac.cwl (CommandLineTool)
name_sorting_filtered_bam
../tools/samtools_sort_name.cwl (CommandLineTool)

Sort a bam file by read names.

peak_calling_macs2_narrow
../tools/macs2_callpeak_atac.cwl (CommandLineTool)
generating_coverage_tracks
generating_atac_signal_tags
../tools/generate_atac_signal_tags.cwl (CommandLineTool)
plot_fragment_size_distribution
../tools/plot_frag_size_distr.cwl (CommandLineTool)

Outputs

ID Type Label Doc
bam File
bowtie2_log File[]
multiqc_zip File
multiqc_html File
raw_fastqc_zip d28ef2b2109c1fe00386a6efc009896c[]
bam_signal_tags File[]
raw_fastqc_html 36a59d50d7c035f7c7d5567f9d98bf1d[]
trim_galore_log ed53163bcb7c907558303f6f39079fff[]
duprem_fastqc_zip File[]
qc_crosscorr_plot File (Optional)
bigwig_signal_tags File[]
duprem_fastqc_html File[]
fragment_sizes_tsv File
picard_markdup_log File
trimmed_fastqc_zip c7d316ae8774b8a1398dd67ed3632d41[]
filtering_stats_tsv File
frag_size_distr_tsv File
frag_size_stats_tsv File
trimmed_fastqc_html 2c1ecaf362e8c8a2a3378c0fbd701a75[]
frag_size_distr_plot File
irreg_mappings_bedpe File
qc_crosscorr_summary File (Optional)
peaks_bed_macs2_broad d6390c5956aec4d366e50552807bcf8d[]
peaks_xls_macs2_broad File[]
duprem_flagstat_output File
merged_flagstat_output File
peaks_bed_macs2_narrow File[]
peaks_xls_macs2_narrow File
qc_plot_fingerprint_tsv File (Optional)
filtered_flagstat_output File
qc_plot_fingerprint_plot File (Optional)
qc_plot_fingerprint_stderr File
qc_phantompeakqualtools_stderr File (Optional)
Permalink: https://w3id.org/cwl/view/git/52515b5c053d3cf157be070efac954043892429f/CWL/workflows/ATACseq_pipeline.cwl