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EMG pipeline's QIIME workflow
Step 1: Set environment PYTHONPATH, QIIME_ROOT, PATH Step 2: Run QIIME script pick_closed_reference_otus.py ${python} ${qiimeDir}/bin/pick_closed_reference_otus.py -i $1 -o $2 -r ${qiimeDir}/gg_13_8_otus/rep_set/97_otus.fasta -t ${qiimeDir}/gg_13_8_otus/taxonomy/97_otu_taxonomy.txt -p ${qiimeDir}/cr_otus_parameters.txt Step 3: Convert new biom format to old biom format (json) ${qiimeDir}/bin/biom convert -i ${resultDir}/cr_otus/otu_table.biom -o ${resultDir}/cr_otus/${infileBase}_otu_table_json.biom --table-type=\"OTU table\" --to-json Step 4: Convert new biom format to a classic OTU table. ${qiimeDir}/bin/biom convert -i ${resultDir}/cr_otus/otu_table.biom -o ${resultDir}/cr_otus/${infileBase}_otu_table.txt --to-tsv --header-key taxonomy --table-type \"OTU table\" Step 5: Create otu summary ${qiimeDir}/bin/biom summarize-table -i ${resultDir}/cr_otus/otu_table.biom -o ${resultDir}/cr_otus/${infileBase}_otu_table_summary.txt Step 6: Move one of the result files mv ${resultDir}/cr_otus/otu_table.biom ${resultDir}/cr_otus/${infileBase}_otu_table_hdf5.biom Step 7: Create a list of observations awk '{print $1}' ${resultDir}/cr_otus/${infileBase}_otu_table.txt | sed '/#/d' > ${resultDir}/cr_otus/${infileBase}_otu_observations.txt Step 8: Create a phylogenetic tree by pruning GreenGenes and keeping observed otus ${python} ${qiimeDir}/bin/filter_tree.py -i ${qiimeDir}/gg_13_8_otus/trees/97_otus.tree -t ${resultDir}/cr_otus/${infileBase}_otu_observations.txt -o ${resultDir}/cr_otus/${infileBase}_pruned.tree |
![]() Path: workflows/qiime-workflow.cwl Branch/Commit ID: 708fd97 |
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chksum_xam_to_interleaved_fq.cwl
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![]() Path: cwls/chksum_xam_to_interleaved_fq.cwl Branch/Commit ID: 0.4.1 |
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count-lines12-wf.cwl
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![]() Path: tests/count-lines12-wf.cwl Branch/Commit ID: main |
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count-lines11-wf-noET.cwl
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![]() Path: tests/count-lines11-wf-noET.cwl Branch/Commit ID: main |
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ValidateTelescopeSimulationModel
Validate the telescope simulation model as a whole. |
![]() Path: workflows/ValidateTelescopeSimulationModel.cwl Branch/Commit ID: main |
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cmsearch-multimodel.cwl
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![]() Path: workflows/cmsearch-multimodel.cwl Branch/Commit ID: 71d9c83 |
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exomeseq-03-organizedirectories.cwl
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![]() Path: subworkflows/exomeseq-03-organizedirectories.cwl Branch/Commit ID: gatk4-fixes |
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dynresreq-workflow-inputdefault.cwl
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![]() Path: v1.0/v1.0/dynresreq-workflow-inputdefault.cwl Branch/Commit ID: master |
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Detect Variants workflow
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![]() Path: detect_variants/detect_variants.cwl Branch/Commit ID: toil_compatibility |
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bwameth_mulitlib_start_with_trimmed.cwl
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![]() Path: workflows/bwameth/bwameth_mulitlib_start_with_trimmed.cwl Branch/Commit ID: main |