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Single-Cell RNA-Seq Cluster Analysis
Single-Cell RNA-Seq Cluster Analysis Clusters cells by similarity of gene expression data from the outputs of the “Single-Cell RNA-Seq Dimensionality Reduction Analysis” pipeline. The results of this workflow are used in the “Single-Cell Manual Cell Type Assignment”, “Single-Cell RNA-Seq Differential Expression Analysis”, “Single-Cell RNA-Seq Trajectory Analysis”, and “Single-Cell Differential Abundance Analysis” pipelines. |
Path: workflows/sc-rna-cluster.cwl Branch/Commit ID: 549fac35bf6b8b1c25af0f4f6c3f162c40dc130e |
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revsort_step_bad_schema.cwl
Reverse the lines in a document, then sort those lines. |
Path: tests/wf/revsort_step_bad_schema.cwl Branch/Commit ID: 6cfef62c21330672538fd5e9b45ec888569c0a6f |
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count-lines11-wf-noET.cwl
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Path: tests/count-lines11-wf-noET.cwl Branch/Commit ID: e515226f8ac0f7985cd94dae4a301150adae3050 |
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kmer_cache_retrieve
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Path: task_types/tt_kmer_cache_retrieve.cwl Branch/Commit ID: 7f9cfcbda5998b164bd1d8f1f6006aefda0f47f3 |
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Motif Finding with HOMER with custom background regions
Motif Finding with HOMER with custom background regions --------------------------------------------------- HOMER contains a novel motif discovery algorithm that was designed for regulatory element analysis in genomics applications (DNA only, no protein). It is a differential motif discovery algorithm, which means that it takes two sets of sequences and tries to identify the regulatory elements that are specifically enriched in on set relative to the other. It uses ZOOPS scoring (zero or one occurrence per sequence) coupled with the hypergeometric enrichment calculations (or binomial) to determine motif enrichment. HOMER also tries its best to account for sequenced bias in the dataset. It was designed with ChIP-Seq and promoter analysis in mind, but can be applied to pretty much any nucleic acids motif finding problem. For more information please refer to: ------------------------------------- [Official documentation](http://homer.ucsd.edu/homer/motif/) |
Path: workflows/homer-motif-analysis-bg.cwl Branch/Commit ID: 549fac35bf6b8b1c25af0f4f6c3f162c40dc130e |
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Chipseq alignment for mouse with qc and creating homer tag directory
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Path: definitions/pipelines/chipseq_alignment_mouse.cwl Branch/Commit ID: a59a803e1809a8fbfabca6b8962a8ad66dd01f1d |
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tt_kmer_compare_wnode
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Path: task_types/tt_kmer_compare_wnode.cwl Branch/Commit ID: ac387721a55fd91df3dcdf16e199354618b136d1 |
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ani_top_n
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Path: task_types/tt_ani_top_n.cwl Branch/Commit ID: 2c7879b47890b9300ab9b5ebd35e17372e077757 |
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AltAnalyze Build Reference Indices
AltAnalyze Build Reference Indices ================================== |
Path: workflows/altanalyze-prepare-genome.cwl Branch/Commit ID: 12e5256de1b680c551c87fd5db6f3bc65428af67 |
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RNA-Seq pipeline paired-end stranded mitochondrial
Slightly changed original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for **strand specific pair-end** experiment. An additional steps were added to map data to mitochondrial chromosome only and then merge the output. Experiment files in [FASTQ](http://maq.sourceforge.net/fastq.shtml) format either compressed or not can be used. Current workflow should be used only with the pair-end strand specific RNA-Seq data. It performs the following steps: 1. `STAR` to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. `fastx_quality_stats` to analyze input FASTQ file and generate quality statistics file 3. `samtools sort` to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using `GEEP` reads-counting utility; export results to file |
Path: workflows/rnaseq-pe-dutp-mitochondrial.cwl Branch/Commit ID: c0ca7b140d776eec223ceb1c620eda17281860c4 |
