Explore Workflows
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EMG pipeline's QIIME workflow
Step 1: Set environment PYTHONPATH, QIIME_ROOT, PATH Step 2: Run QIIME script pick_closed_reference_otus.py ${python} ${qiimeDir}/bin/pick_closed_reference_otus.py -i $1 -o $2 -r ${qiimeDir}/gg_13_8_otus/rep_set/97_otus.fasta -t ${qiimeDir}/gg_13_8_otus/taxonomy/97_otu_taxonomy.txt -p ${qiimeDir}/cr_otus_parameters.txt Step 3: Convert new biom format to old biom format (json) ${qiimeDir}/bin/biom convert -i ${resultDir}/cr_otus/otu_table.biom -o ${resultDir}/cr_otus/${infileBase}_otu_table_json.biom --table-type=\"OTU table\" --to-json Step 4: Convert new biom format to a classic OTU table. ${qiimeDir}/bin/biom convert -i ${resultDir}/cr_otus/otu_table.biom -o ${resultDir}/cr_otus/${infileBase}_otu_table.txt --to-tsv --header-key taxonomy --table-type \"OTU table\" Step 5: Create otu summary ${qiimeDir}/bin/biom summarize-table -i ${resultDir}/cr_otus/otu_table.biom -o ${resultDir}/cr_otus/${infileBase}_otu_table_summary.txt Step 6: Move one of the result files mv ${resultDir}/cr_otus/otu_table.biom ${resultDir}/cr_otus/${infileBase}_otu_table_hdf5.biom Step 7: Create a list of observations awk '{print $1}' ${resultDir}/cr_otus/${infileBase}_otu_table.txt | sed '/#/d' > ${resultDir}/cr_otus/${infileBase}_otu_observations.txt Step 8: Create a phylogenetic tree by pruning GreenGenes and keeping observed otus ${python} ${qiimeDir}/bin/filter_tree.py -i ${qiimeDir}/gg_13_8_otus/trees/97_otus.tree -t ${resultDir}/cr_otus/${infileBase}_otu_observations.txt -o ${resultDir}/cr_otus/${infileBase}_pruned.tree |
![]() Path: workflows/qiime-workflow.cwl Branch/Commit ID: 8e196ab |
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revsort.cwl
Reverse the lines in a document, then sort those lines. |
![]() Path: examples/revsort-run-1/snapshot/revsort.cwl Branch/Commit ID: main |
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Whole Exome Sequencing
Whole Exome Sequence analysis using GATK best practices - Germline SNP & Indel Discovery |
![]() Path: packed/exomeseq.cwl Branch/Commit ID: qiime2-workflow Packed ID: main |
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BD Rhapsody™ Targeted Analysis Pipeline
The BD Rhapsody™ assays are used to create sequencing libraries from single cell transcriptomes. After sequencing, the analysis pipeline takes the FASTQ files and a reference file for gene alignment. The pipeline generates molecular counts per cell, read counts per cell, metrics, and an alignment file. |
![]() Path: v1.8/rhapsody_targeted_1.8.cwl Branch/Commit ID: master Packed ID: main |
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ST520106.cwl
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![]() Path: wf5201/ST520106.cwl Branch/Commit ID: main |
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collate_unique_rRNA_headers.cwl
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![]() Path: tools/collate_unique_rRNA_headers.cwl Branch/Commit ID: ef3c7b2 |
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Alignment without BQSR
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![]() Path: definitions/subworkflows/sequence_to_bqsr_nonhuman.cwl Branch/Commit ID: low-vaf |
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gathered exome alignment and somatic variant detection
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![]() Path: definitions/pipelines/somatic_exome_gathered.cwl Branch/Commit ID: low-vaf |
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presto.cwl
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![]() Path: presto.cwl Branch/Commit ID: visualise |
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kfdrc_alignment_CramOnly_wf.cwl
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![]() Path: workflows/kfdrc_alignment_CramOnly_wf.cwl Branch/Commit ID: dev |