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DESeq - differential gene expression analysis
Differential gene expression analysis ===================================== Differential gene expression analysis based on the negative binomial distribution Estimate variance-mean dependence in count data from high-throughput sequencing assays and test for differential expression based on a model using the negative binomial distribution. DESeq1 ------ High-throughput sequencing assays such as RNA-Seq, ChIP-Seq or barcode counting provide quantitative readouts in the form of count data. To infer differential signal in such data correctly and with good statistical power, estimation of data variability throughout the dynamic range and a suitable error model are required. Simon Anders and Wolfgang Huber propose a method based on the negative binomial distribution, with variance and mean linked by local regression and present an implementation, [DESeq](http://bioconductor.org/packages/release/bioc/html/DESeq.html), as an R/Bioconductor package DESeq2 ------ In comparative high-throughput sequencing assays, a fundamental task is the analysis of count data, such as read counts per gene in RNA-seq, for evidence of systematic changes across experimental conditions. Small replicate numbers, discreteness, large dynamic range and the presence of outliers require a suitable statistical approach. [DESeq2](http://www.bioconductor.org/packages/release/bioc/html/DESeq2.html), a method for differential analysis of count data, using shrinkage estimation for dispersions and fold changes to improve stability and interpretability of estimates. This enables a more quantitative analysis focused on the strength rather than the mere presence of differential expression. |
![]() Path: workflows/deseq.cwl Branch/Commit ID: master |
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step-valuefrom-wf.cwl
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![]() Path: tests/step-valuefrom-wf.cwl Branch/Commit ID: main |
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contamination_foreign_chromosome
This workflow detect and remove foreign chromosome from a DNA fasta file |
![]() Path: workflows/Contamination/contamination-foreign-chromosome-blastn.cwl Branch/Commit ID: master |
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io-file-default-wf.cwl
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![]() Path: tests/io-file-default-wf.cwl Branch/Commit ID: main |
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waltz_workflow_all_bams.cwl
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![]() Path: workflows/waltz/waltz_workflow_all_bams.cwl Branch/Commit ID: master |
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Functional analyis of sequences that match the 16S SSU
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![]() Path: workflows/16S_taxonomic_analysis.cwl Branch/Commit ID: 9c57dba |
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env-wf1.cwl
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![]() Path: tests/env-wf1.cwl Branch/Commit ID: main |
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cond-wf-002.cwl
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![]() Path: tests/conditionals/cond-wf-002.cwl Branch/Commit ID: main |
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minibam_sub_wf.cwl
This is a subworkflow of the main oxog_varbam_annotat_wf workflow - this is not meant to be run as a stand-alone workflow! |
![]() Path: minibam_sub_wf.cwl Branch/Commit ID: master |
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work9.cwl
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![]() Path: work9.cwl Branch/Commit ID: main |