Explore Workflows
View already parsed workflows here or click here to add your own
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count-lines4-wf.cwl
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![]() Path: tests/count-lines4-wf.cwl Branch/Commit ID: master |
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alignment_workflow_md5checker.cwl
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![]() Path: aligner/topmed-cwl/workflow/alignment_workflow_md5checker.cwl Branch/Commit ID: 1.32.0 |
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validate_interleaved_fq.cwl
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![]() Path: cwls/validate_interleaved_fq.cwl Branch/Commit ID: 0.5.0_test |
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Metagenomic Binning from Assembly
Workflow for Metagenomics from raw reads to annotated bins.<br> Summary - MetaBAT2 (binning) - CheckM (bin completeness and contamination) - GTDB-Tk (bin taxonomic classification) - BUSCO (bin completeness) **All tool CWL files and other workflows can be found here:**<br> Tools: https://git.wur.nl/unlock/cwl/-/tree/master/cwl<br> Workflows: https://git.wur.nl/unlock/cwl/-/tree/master/cwl/workflows<br> The dependencies are either accessible from https://unlock-icat.irods.surfsara.nl (anonymous,anonymous)<br> and/or<br> By using the conda / pip environments as shown in https://git.wur.nl/unlock/docker/-/blob/master/kubernetes/scripts/setup.sh<br> |
![]() Path: cwl/workflows/workflow_metagenomics_binning.cwl Branch/Commit ID: master |
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bulk-atac-seq-pipeline.cwl
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![]() Path: bulk-atac-seq-pipeline.cwl Branch/Commit ID: develop |
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Runs InterProScan on batches of sequences to retrieve functional annotations.
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![]() Path: workflows/InterProScan-v5-chunked-wf.cwl Branch/Commit ID: cwlexec |
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SSU-from-tablehits.cwl
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![]() Path: tools/SSU-from-tablehits.cwl Branch/Commit ID: 3168316 |
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preference-workflow.cwl
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![]() Path: predict_service/preference-workflow.cwl Branch/Commit ID: master |
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Trim Galore RNA-Seq pipeline single-read
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **single-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-end RNA-Seq data. It performs the following steps: 1. Trim adapters from input FASTQ file 2. Use STAR to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 3. Use fastx_quality_stats to analyze input FASTQ file and generate quality statistics file 4. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
![]() Path: workflows/trim-rnaseq-se.cwl Branch/Commit ID: master |
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rRNA_selection.cwl
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![]() Path: tools/rRNA_selection.cwl Branch/Commit ID: 43d2fb8 |