Workflow: Raw sequence data to BQSR
Fetched 2023-01-03 22:52:03 GMT
Verified with cwltool version 3.1.20221201130942
- Selected
- |
- Default Values
- Nested Workflows
- Tools
- Inputs/Outputs
This workflow is Open Source and may be reused according to the terms of:
MIT License
Note that the tools invoked by the workflow may have separate licenses.
Inputs
| ID | Type | Title | Doc |
|---|---|---|---|
| trimming | https://w3id.org/cwl/view/git/da335d9963418f7bedd84cb2791a0df1b3165ffe/definitions/types/trimming_options.yml#trimming_options (Optional) | ||
| reference | File | ||
| unaligned | https://w3id.org/cwl/view/git/da335d9963418f7bedd84cb2791a0df1b3165ffe/definitions/types/sequence_data.yml#sequence_data[] | ||
| final_name | String | ||
| bqsr_intervals | String[] (Optional) | ||
| bqsr_known_sites | File[] |
One or more databases of known polymorphic sites used to exclude regions around known polymorphisms from analysis. |
Steps
| ID | Runs | Label | Doc |
|---|---|---|---|
| bqsr |
../tools/bqsr.cwl
(CommandLineTool)
|
create BQSR table | |
| align |
sequence_align_and_tag_adapter.cwl
(Workflow)
|
adapter for sequence_align_and_tag |
Some workflow engines won't stage files in our nested structure, so parse it out here |
| merge |
../tools/merge_bams_samtools.cwl
(CommandLineTool)
|
Samtools: merge | |
| index_bam |
../tools/index_bam.cwl
(CommandLineTool)
|
samtools index | |
| name_sort |
../tools/name_sort.cwl
(CommandLineTool)
|
sort BAM by name | |
| apply_bqsr |
../tools/apply_bqsr.cwl
(CommandLineTool)
|
apply BQSR | |
| mark_duplicates_and_sort |
../tools/mark_duplicates_and_sort.cwl
(CommandLineTool)
|
Mark duplicates and Sort |
Outputs
| ID | Type | Label | Doc |
|---|---|---|---|
| final_bam | File | ||
| mark_duplicates_metrics_file | File |
Permalink:
https://w3id.org/cwl/view/git/da335d9963418f7bedd84cb2791a0df1b3165ffe/definitions/subworkflows/sequence_to_bqsr.cwl
